Efficient clonal propagation of Rangpur lime (Citrus limonia) using mature nodal explants: An evaluation of lab to land production of disease-free plants

Volume 1, Issue 1 (Jun-Aug 2025)

Authors: Sangeeta Sen and Vibha Dhawan

DOI: doi.org/10.64848/WJST.1.1.2025.44-56

Page No: 44-56

Keywords: Micropropagation, Axillary branching, Rangpur lime, Rootstock, Clonal fidelity, ISSR

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Abstract

Rangpur lime is one of the most promising Citrus rootstocks for Indian conditions. An efficient micropropagation protocol from mature nodal explants of Rangpur lime was developed using the enhanced axillary branching method. In the present study, multiple shoots were obtained by stimulating axillary shoots from single nodes derived from field-grown trees on Murashige and Skoog’s (MS) medium containing BA (1.11 µM), Kinetin (1.16 µM), and 3% sucrose and 100% rooting was obtained in half-strength MS medium. The rooted plants were then transferred to a potting mix containing soil and agropeat. Once again, 100% survival was observed during acclimatisation. Inter simple sequence repeats (ISSR) analysis of the in vitro derived progeny was used to determine their clonal fidelity of the regenerants vis-à-vis the mother plant. The results of this study have enormous commercial applications for the propagation of clean and healthy disease-free citrus rootstocks.